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KMID : 0380019940090050532
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Korean Journal of Biotechnology and Bioengineering
1994 Volume.9 No. 5 p.532 ~ p.539
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Secretion Characteristics of Foreign Glucoamylase from Recombinant Plasmid-Harboring and Chromosome-Integrated Saccharomyces cerevisiaes
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차형준/Cha HJ
조광명/유영제/Cho KM/Yoo YJ
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Abstract
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Secretion efficiency is generally affected by promote;signal sequence, characteristics of foreign protein and host. Secretion efficiencies of glucoamylase in recombinant plasmid-harboring yeast and chromosome-integrated yeast which had STA signal sequences were 74% and 65% at the 4th day of incubation, respectively. The high secretion efficiencies of the yeasts were obtained due to the fact that the expression levels were not reached at the secretory apparatus capacities of the host yeasts. In both yeasts, most of the intracellular glucoamylase were detected in cytoplasm and small portion (below 10%) of glucoamylase were located in periplasm. The characteristics of secreted heterologous glucoarnylase from recombinant Saccharomyces cerevisiae were investigated by using Western blot analysis. The secreted mature glucoamylase was heterogeneous and its molecular weight was about 200 to 300 kilodalton. The carbohydrate content of mature glucoamylase was higher than 80%, and several bands of about 55 to 65 kilodalton indicate the endoplasmic reticulum forms of intracellular glucoamylase.
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KEYWORD
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